The second round of population testing for the presence of COVID-19 disease will be carried out differently. By decision of the Central Crisis Headquarters of the Slovak Republic, the district will be exempted with a number of positive cases below 0.7 percent. It will also not be tested in Bratislava and Košice. In total 45 districts will be tested during 7 and 8 November 2020. In view of the fact that pilot testing and also the first round of sweeping testing have proved to be effective tools in order to prevent the spread of the disease among our inhabitants, it is important not to let go. We need to stabilise all regions and we cannot be satisfied with the good situation in some parts of Slovakia. I mean, as they say, one battle doesn't win a war reasoned minister for Defence of SR Jaroslav Come on with the fact that he sincerely believes in the understanding and support of all those involved in the testing and also of the residents who are to participate in it.List of tested districts:
| Regional Commands | Landkreises |
| Malacky | Senica |
| Skalica | |
| Martin | Dolný Kubín |
| Martin | |
| Square | |
| Turčianske Teplice | |
| Michalovce | Humenne |
| Interlaborators | |
| Michalovce | |
| Snina | |
| Sobrance | |
| Vranov nad Topľou | |
| Prešov | Bardejov |
| Kežmarok | |
| Levoča | |
| Poprad | |
| Prešov | |
| Sabinov | |
| Old Lubovňa | |
| Ceiling | |
| Svidník | |
| Rožňava | Gelnica |
| Spišská Nová Ves | |
| Ružomberok | Liptovský Mikulas |
| Ružomberok | |
| Hardaches | |
| Sered | Danube Wednesday |
| Topoľčany | Partisan |
| Prievidza | |
| Topoľčany | |
| Trencin | Bánovce nad Bebravou |
| Ilava | |
| Myjava | |
| New City n/ V | |
| Považská Bystrica | |
| Reeds | |
| Trencin | |
| Selected | Banská Bystrica |
| Brezno | |
| Detva | |
| Selected | |
| Žilina | Bytča |
| Chadca | |
| Kysucké New City | |
| Žilina | |
| TOTAL | 45 counties |